2(5H)-Furanone is an endogenously produced metabolite.

Physicochemical Properties

Molecular Formula	C4H4O2
Molecular Weight	84.07
Exact Mass	84.021
CAS #	497-23-4
PubChem CID	10341
Appearance	Colorless to light yellow liquid
Density	1.2±0.1 g/cm3
Boiling Point	203.7±0.0 °C at 760 mmHg
Melting Point	4-5 °C(lit.)
Flash Point	101.1±0.0 °C
Vapour Pressure	0.3±0.4 mmHg at 25°C
Index of Refraction	1.481
LogP	-0.84
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	0
Heavy Atom Count	6
Complexity	93.7
Defined Atom Stereocenter Count	0
SMILES	O1C(C([H])=C([H])C1([H])[H])=O
InChi Key	VIHAEDVKXSOUAT-UHFFFAOYSA-N
InChi Code	InChI=1S/C4H4O2/c5-4-2-1-3-6-4/h1-2H,3H2
Chemical Name	2H-furan-5-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Human Endogenous Metabolite
Additional Infomation	But-2-en-4-olide is a butenolide. It is a tautomer of a but-3-en-4-olide. 2(5H)-Furanone has been reported in Camellia sinensis, Fusarium graminearum, and other organisms with data available. See also: Butenolide (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (1189.48 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (29.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (29.74 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (29.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	11.8948 mL	59.4742 mL	118.9485 mL
	5 mM	2.3790 mL	11.8948 mL	23.7897 mL
	10 mM	1.1895 mL	5.9474 mL	11.8948 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.