| Bioactivity | WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm[1]. Storage: protect from light. |
| Invitro | WST-1 工作液的配制1.1 制备储存液用 DMSO 配制 10 mg/mL 的 WST-1。如用1 mL DMSO 溶解 10 mg WST-1。注：WST-1 储存液建议分装后于 -20 ℃ 或 -80 ℃ 避光保存。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 5mM 的 WST-1 工作液。注：请根据实际情况调整 WST-1 工作液浓度，且现用现配。细胞染色2.1 准备工作悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 加入 1 mL WST-1 工作液和1-methoxy PMS (2 mM)，室温孵育 2 h。2.3 用酶标仪测定 450 和 690 nm 处光密度。 |
| Name | WST-1 |
| CAS | 150849-52-8 |
| Formula | C19H11IN5NaO8S2 |
| Molar Mass | 651.34 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Munetaka ISHIYAMA, et al. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. 1993, 41(6): 1118-1122. |
WST-1
CAS: 150849-52-8 F: C19H11IN5NaO8S2 W: 651.34
WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct
Sales Email：peptidedb@qq.com
This product is for research use only, not for human use. We do not sell to patients.