| Bioactivity | MQAE is a novel chloride ion fluorescence probe excited by ultraviolet light. MQAE, binding bromine ion as ligand, can be used to detect the activity of chloride. MQAE exhibits a reduction of fluorescence intensity with the increase of intracellular Cl- concentration. MQAE has a pH-independent fluorescence intensity basically at a Cl- concentration <50 nM. MQAE has high water solubility and cell permeability, MQAE has been used for fluorescence detection such as confocal microscopy and flow cytometry. Ex/Em=350/460 nm[1]. Storage: protect from light. |
| Invitro | 1.MQAE 工作液配制1.1 制备储存液将 1 mg MQAE 用 0.3066 mL DMSO 配制 10 mM 储备液。1.2 工作液的配制使用 Krebs-HEPES 缓冲液 (20 mM HEPES，128 mM NaCl，2.5 mM KCl，2.7 mM CaCl2，1 mM MgCl2，16 mM glucose，pH 7.4) 配制 5-10 μM 的 MQAE 工作液。注：请根据实际情况调整 MQAE 工作液浓度，且现用现配。2 细胞染色2.1 准备工作悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 加入 1 mL MQAE 工作液，室温孵育 30 分钟。2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜进行观察。 |
| Name | MQAE |
| CAS | 162558-52-3 |
| Formula | C14H16BrNO3 |
| Molar Mass | 326.19 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| Reference | [1]. Kovalchuk Y, et al. Two-photon chloride imaging using MQAE in vitro and in vivo. Cold Spring Harb Protoc. 2012 Jul 1;2012(7):778-85. [2]. Andersson C, et al. Determination of chloride efflux by X-ray microanalysis versus MQAE-fluorescence. Microsc Res Tech. 2 [3]. Koncz C, et al. Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am J Physiol. 1994 Dec;267(6 Pt 2):H2114-23. |