DiD perchlorate_product_DataBase

Bioactivity	DiD is a long-chain carbocyanine dye. Carbocyanine dyes are widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins[2].
Invitro	制备 Di 染色溶液1.1 制备 DMF、DMSO 或乙醇储备溶液：储备溶液应在 1-5 mM 的二甲基甲酰胺 (DMF) 、二甲基亚砜 (DMSO 或乙醇 DMSO 中制备。DMF 比乙醇更适合作为 Di 的溶剂。储备溶液应及时使用。任何未使用的溶液可作分装储存在 -20℃。避免反复冻融，溶液可储存 6 个月。1.2 制备工作溶液：将储备溶液稀释到合适的缓冲液中，如无血清培养基、HBS 或 PBS，以制备 1-5 μM工作溶液，现配现用。注：应根据经验确定不同实验条件下工作溶液的最终浓度。2. 悬浮细胞2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在 1×106/mL2.2 加入 1 mL Di 工作液，室温孵育 5-30 分钟。2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。3. 贴壁细胞3.1 将贴壁细胞培养于无菌盖玻片上。3.2 从培养基中移出盖玻片，吸除多余培养基。3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 分钟。3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟，使用荧光显微镜或流式细胞仪进行观察。
In Vivo	Two weeks after injection of stained cells, a single, bright, DiD perchlorate (DiD)-positive cells located between 15 to 40 µm from the endosteum is found. Results reveal a progressive appearance of cell clusters of decreased dye intensity, consistent with the partitioning of DiD perchlorate label on cell division[3].
Name	DiD perchlorate
CAS	127274-91-3
Formula	C61H99ClN2O4
Molar Mass	959.90
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	4°C, protect from light, stored under nitrogen *该产品在溶液状态不稳定，建议您现用现配，即刻使用。
Reference	[1]. Gan WB, et al. Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron. 2000 Aug;27(2):219-25. [2]. Kenji Yumoto, et al. A novel method for monitoring tumor dormancy using fluorescent dye DiD. Cytometry A. 2014 Jun; 85(6): 548–555. [3]. Meng Li, et al. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J Vis Exp. 2017; (128): 56273. [4]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.

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DiD perchlorate

CAS: 127274-91-3 F: C61H99ClN2O4 W: 959.90