Physicochemical Properties
| Molecular Formula | C18H17NO3 |
| Molecular Weight | 295.33 |
| Exact Mass | 295.121 |
| CAS # | 517-71-5 |
| PubChem CID | 160503 |
| Appearance | Light yellow to yellow solid powder |
| Density | 1.289g/cm3 |
| Boiling Point | 483.4ºC at 760mmHg |
| Flash Point | 193.7ºC |
| Index of Refraction | 1.635 |
| LogP | 3.162 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 4 |
| Rotatable Bond Count | 1 |
| Heavy Atom Count | 22 |
| Complexity | 431 |
| Defined Atom Stereocenter Count | 1 |
| SMILES | COC1=CC2=C(C=C1)C3=C4[C@@H](C2)NCCC4=CC5=C3OCO5 |
| InChi Key | RFWCCZDSXIZJMF-CQSZACIVSA-N |
| InChi Code | InChI=1S/C18H17NO3/c1-20-12-2-3-13-11(6-12)7-14-16-10(4-5-19-14)8-15-18(17(13)16)22-9-21-15/h2-3,6,8,14,19H,4-5,7,9H2,1H3/t14-/m1/s1 |
| Chemical Name | (12R)-16-methoxy-3,5-dioxa-11-azapentacyclo[10.7.1.02,6.08,20.014,19]icosa-1(20),2(6),7,14(19),15,17-hexaene |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | Xylopine exhibits strong cytotoxicity in a dose- and time-dependent manner (3.5 μM–14 μM; 24-48 hours)[1]. With IC50 values ranging from 6.4 to 26.6 μM in eight distinct cancer cell lines (MCF7, HCT116, HepG2, SCC-9, HSC-3, HL-60, K-562, and B16-F10), xylopine (72 h) has cytotoxic activity[1]. Internucleosomal DNA fragmentation follows cell cycle arrest at phase G2/M caused by xylopine (3.5 μM–14 μM; 24-48 hours)[1]. 3.5 μM–14 μM; 24-48 hours) of xylopine significantly enhances caspase-3 activation, produces mitochondrial depolarization, and increases early and late apoptosis[1]. 1]. G2/M phase arrest was induced in HCT116 cells[1] at 3.5 μM, 7 μM, and 14 μM for 24 and 48 hours. In HCT116 cells[1], the early and late apoptosis was significantly increased by 3.5 μM, 7 μM, and 14 μM after 24 and 48 hours. |
| Cell Assay |
Cell Viability Assay[1] Cell Types: HCT116 cells Tested Concentrations: 3.5 μM, 7 μM, and 14 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: Displayed potent cytotoxicity in HCT116 cells. Cell Cycle Analysis[1] Cell Types: HCT116 cells Tested Concentrations: 3.5 μM, 7 μM, and 14 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: Induced G2 /M phase arrest. Apoptosis Analysis[1] Cell Types: HCT116 cells Tested Concentrations: 3.5 μM, 7 μM, and 14 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: Dramatically increased the early and late apoptosis. |
| References |
[1]. Xylopine Induces Oxidative Stress and Causes G 2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells. Oxid Med Cell Longev. 2017;2017:7126872. |
| Additional Infomation |
Xylopine is an aporphine alkaloid. Xylopine has been reported in Magnolia liliifera, Xylopia parviflora, and other organisms with data available. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3860 mL | 16.9302 mL | 33.8604 mL | |
| 5 mM | 0.6772 mL | 3.3860 mL | 6.7721 mL | |
| 10 mM | 0.3386 mL | 1.6930 mL | 3.3860 mL |