EGFR-IN-140 (Compound 31) is an inhibitor of EGFR, inhibiting EGFR wild-type and EGFR L858R/T790M/C797S mutant with Ki of 0.95 nM and 2.1 nM, respectively, and inhibiting EGFR del19/T790M/C797S in Ba/F3 with IC50 of 56.9 nM. EGFR-IN-140 exhibits antitumor activity in mouse models.

Physicochemical Properties

Molecular Formula	C27H37FN8O2
Molecular Weight	524.63
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	EGFR 0.95 nM (Ki); EGFRL858R/T790M/C797S 2.1 nM (Ki)
References	[1]. Discovery of a Novel Mutant-Selective Epidermal Growth Factor Receptor Inhibitor Using an In Silico Enabled Drug Discovery Platform. J Med Chem. 2024 Dec 26;67(24):21811-21840.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9061 mL	9.5305 mL	19.0611 mL
	5 mM	0.3812 mL	1.9061 mL	3.8122 mL
	10 mM	0.1906 mL	0.9531 mL	1.9061 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.