| Bioactivity | iNOS/COX-2-IN-1 (Compound 12e) is an inhibitor of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). iNOS/COX-2-IN-1 inhibits the NF-κB and MAPKs signaling pathways and thus exerts anti-inflammatory effects[1]. |
| Invitro | iNOS/COX-2-IN-1 (0.625-40 μM; 24 h) 在 5 μM 浓度下对 RAW 264.7 细胞无明显细胞毒性[1]。iNOS/COX-2-IN-1 (2.5-10 μM; 1 h) 显著抑制了 LPS 诱导的 NO 生成，NO 抑制率为 45.38 % (5 μM)。浓度依赖性地抑制了 TNF-α 和 COX-2 的表达。效抑制促炎性细胞因子的 mRNA 表达。通过抑制 NF-κB 信号通路的激活，发挥其抗炎作用[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> iNOS/COX-2-IN-1 相关抗体: Cell Cytotoxicity Assay[1] Cell Line: |
| Formula | C31H38FN3O2S |
| Molar Mass | 535.72 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Cai X, et al. Design, synthesis and molecular modeling of novel D-ring substituted steroidal 4,5-dihydropyrazole thiazolinone derivatives as anti-inflammatory agents by inhibition of COX-2/iNOS production and down-regulation of NF-κB/MAPKs in LPS-induced RAW264.7 macrophage cells. Eur J Med Chem. 2024 Apr 27;272:116460. |