| Bioactivity | Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1]. |
| Invitro | 1.TMRE 工作液的配制1.1 制备储存液用 525 μL 无水 DMSO 稀释 1 mg TMRE 去配制 5 mM 的储备液。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-20 μM 的 TMRE 工作液。注：请根据实际情况调整 TMRE 工作液浓度，且现用现配。2. 细胞染色(悬浮细胞)2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在1×106/mL。2.2 加入 1 mL TMRE 工作液，室温孵育 30-60 分钟。2.3 400 g，离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。3. 细胞染色(贴壁细胞)3.1 将贴壁细胞培养于无菌盖玻片上。3.2 从培养基中移出盖玻片，吸除多余培养基。3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 分钟。3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟 ，使用荧光显微镜或流式细胞仪进行观察。 |
| Name | TMRE |
| CAS | 115532-52-0 |
| Formula | C26H27ClN2O7 |
| Molar Mass | 514.95 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| Reference | [1]. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361. [2]. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361. [3]. Bantseev V, et al. Confocal laser scanning microscopy imaging of dynamic TMRE movement in the mitochondria of epithelial and superficial cortical fiber cells of bovine lenses. Mol Vis. 2005 Jul 14;11:518-23. |