| Bioactivity | Fluo-4 AM is a?cell-permeable Ca2+?indicator[1]. Storage: protect from light. |
| Invitro | Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright[1].Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1.Count the cells and take 106 cells from each sample (control and experiment/s).2.Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.3.Resuspend the cells in 0.5-ml PBS and add 0.5 μl of Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 °C for 1 h.4.Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1-ml PBS. Separate into 2 flow cytometry tubes—0.5 ml in each.5.Evaluate the staining by flow cytometry and analyze the data by a software such as CellQuest software. |
| Name | Fluo-4 AM |
| CAS | 273221-67-3 |
| Formula | C51H50F2N2O23 |
| Molar Mass | 1096.94 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture and light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。 |
| Reference | [1]. Gee KR, et al. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106. [2]. Fluo-4. |