| Bioactivity | TAT-CIRP is a a small peptide, refers to Trans-trans-activating (Tat)-cold-inducible RNA binding protein. TAT-CIRP is an inhibitor of myeloid differentiation protein 2 (MD2). TAT-CIRP exhibits robust neuroprotection against ischemic and hemorrhagic stroke in mice[1]. | ||||||
| Invitro | TAT-CIRP (5 μM) 通过 TLR4 非依赖性途径，减轻神经细胞中 NMDA 刺激（50 μM；1 小时）诱导的细胞凋亡和坏死[1]。 | ||||||
| In Vivo | TAT-CIRP (5-20mg/kg) 减轻大脑中动脉 (MCA) 闭塞 (MCAO) 小鼠缺血性中风后的脑损伤。至于 Tat，人类免疫缺陷病毒 1 型的细胞膜转导结构域，可用作对照肽[1]。TAT-CIRP (20 mg/kg；静脉注射；单剂量) 在小鼠中可通过血脑屏障，Cmax 值为 4762.6 μg/L，半衰期约为 90 分钟[1]。TAT-CIRP (20 mg/kg、100 mg/kg；静脉注射；每天一次，持续 7 天) 对小鼠几乎没有毒性，具有安全性[1]。TAT-CIRP 减少恒河猴的脑缺血损伤[1]。 Animal Model: | ||||||
| Name | TAT-CIRP | ||||||
| Sequence | Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Arg-Gly-Phe-Ser-Arg-Gly-Gly-Gly-Asp-Arg-Gly-Tyr-Gly-Gly | ||||||
| Shortening | YGRKKRRQRRRGRGFSRGGGDRGYGG | ||||||
| Formula | C123H206N56O33 | ||||||
| Molar Mass | 2997.31 | ||||||
| Appearance | Solid | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage | Sealed storage, away from moisture and light, under nitrogen
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen) |
||||||
| Reference | [1]. Fang Z, et al. An MD2-perturbing peptide has therapeutic effects in rodent and rhesus monkey models of stroke. Sci Transl Med. 2021 Jun 9;13(597):eabb6716. |