| Bioactivity | SBFI is a membrane-permeant, fluorescent Na+ indicator dye. SBFI is excited at 340 nm and the fluorophore emission is collected at 450 nm[1]. SBFI selective for Na+ over K+ with Kd values of 20 and 120 mM for these ions, respectively. [2]. |
| Invitro | 每个样品的 SBFI 染料的制备方法如下：[1]1.将适量的染料粉末溶解在 3mL 二甲基亚砜中。2.将五种溶液搅拌 5 分钟。3. 然后用 Leitz Orthoplan 显微镜将每个样品暴露在紫外光下，并记录荧光水平。4. 每个溶液总共获得十个读数，在读数之间搅拌。5. 对于每组样品结果，根据染料浓度绘制最高值、最低值和中值。6. 根据这些测试，使用 50 μg SBFI 染料确定了上限和下限变化最小的最佳荧光值。 |
| Name | SBFI |
| CAS | 124549-08-2 |
| Formula | C44H42N2O15 |
| Molar Mass | 838.81 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Leeves MA, The effect of mechanical deformation on the distribution of ions in fibroblasts. Am J Orthod Dentofacial Orthop. 1995 Jun;107(6):625-32. [2]. Minta A, Fluorescent indicators for cytosolic sodium. J Biol Chem. 1989 Nov 15;264(32):19449-57. |