NSP-SA-NHS_product_DataBase

Bioactivity	NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed[1]. Storage: protect from light.
Invitro	NSP-SA-NHS 标记[1](1) 1mg BSA 溶于 0.3 mL PBS 中 (A 溶液)，0.2 mg NSP-SA-NHS 溶于 40 µL DMF 中 (B 溶液)。(2) 将 B 溶液加入 A 溶液中，18°C、130 rpm、暗处混合 12 h。(3) 混合液与 500 mL 蒸馏水在 4°C 下透析 2 天，期间换 2 次蒸馏水 (C 溶液)。(4) 将 B 溶液缓慢加入 C 溶液中，18°C、130 rpm、暗处混合 24 h，然后用蒸馏水透析 48 h，期间换 4 次蒸馏水。(5) 用蒸馏水稀释偶联物至合适浓度，用紫外分光光度计进行分析。
Name	NSP-SA-NHS
CAS	199293-83-9
Formula	C32H31N3O10S2
Molar Mass	681.73
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	-20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Reference	[1]. Fan Fan Yang, et al. A Rapid and Sensitive Chemiluminescent Immunoassay of Bisphenol a with NSP-SA-NHS-Labeled. Applied Mechanics & Materials, 2014, 707:7-11.

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