Bioactivity | NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed[1]. Storage: protect from light. |
Invitro | NSP-SA-NHS 标记[1](1) 1mg BSA 溶于 0.3 mL PBS 中 (A 溶液),0.2 mg NSP-SA-NHS 溶于 40 µL DMF 中 (B 溶液)。(2) 将 B 溶液加入 A 溶液中,18°C、130 rpm、暗处混合 12 h。(3) 混合液与 500 mL 蒸馏水在 4°C 下透析 2 天,期间换 2 次蒸馏水 (C 溶液)。(4) 将 B 溶液缓慢加入 C 溶液中,18°C、130 rpm、暗处混合 24 h,然后用蒸馏水透析 48 h,期间换 4 次蒸馏水。(5) 用蒸馏水稀释偶联物至合适浓度,用紫外分光光度计进行分析。 |
Name | NSP-SA-NHS |
CAS | 199293-83-9 |
Formula | C32H31N3O10S2 |
Molar Mass | 681.73 |
Appearance | Solid |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | -20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
Reference | [1]. Fan Fan Yang, et al. A Rapid and Sensitive Chemiluminescent Immunoassay of Bisphenol a with NSP-SA-NHS-Labeled. Applied Mechanics & Materials, 2014, 707:7-11. |