| Bioactivity | TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm). |
| Invitro | In contact with cells, TMA-DPH is instantaneously incorporated into the plasma membrane. TMA-DPH is concentrated in lysosomes and highly acidic late endosomes[1]. The TMA-DPH fluorescence lifetime remains constant, 6.2±0.2 ns, below the same critical concentration of 2 μM, but decreases significantly with higher concentrations. This decrease, however, slows down above 5 μM. The TMA-DPH fluorescence anisotropy displays a similar evolution to the fluorescence lifetime. It is first constant, 0.283±0.003, again until 2 μM, and then decreases rapidly to 0.270±0.003 with 5 μM, continuing to fall, but at a lower rate with higher concentrations[2]. |
| Name | TMA-DPH |
| CAS | 115534-33-3 |
| Formula | C28H31NO3S |
| Molar Mass | 461.62 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture and light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。 |
| Reference | [1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94. [2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66. |