| Bioactivity | NLRP3-IN-33 (Compound 12o) is a blood-brain barrier permeable inhibitor of AChE and BChE, with IC50 values of 1.02 μM and 7.03 μM against hAChE and hBChE respectively. NLRP3-IN-33 possesses antioxidant, anti-inflammatory, and metal chelating activities, making it a potential candidate for research in Alzheimer's disease (AD)[1]. |
| Target | IC50: 1.02 Μm (hAChE).IC50: 7.03 μM (hBChE). |
| Invitro | NLRP3-IN-33 (12o) (1-30 μM; 24 h) 在 PC-12 细胞中没有明显的细胞毒作用[1]。NLRP3-IN-33 具有抗氧化活性，能抑制自由基的产生，IC50 值为 6.19 μM，12o (1-20 μM; 24 h) 在 PC-1 细胞有效减轻 H2O2 (600 μM; 24 h) 诱导的氧化应激具有神经保护作用[1。NLRP3-IN-33 (1-20 μM; 24 h) 在 PC-1 细胞中还抑制了 NLRP3 炎症小体的激活，并减轻了由 LPS (1 μg/mL) 和 ATP (5 mM) 在 HMC-3 细胞中引起的线粒体诱导的活性氧 (ROS) 和线粒体膜电位 (MMP) 损伤[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> NLRP3-IN-33 相关抗体: Western Blot Analysis[1] Cell Line: |
| In Vivo | NLRP3-IN-33 (12o) (0.05-0.02 mg/mL) 在低剂量下 (0.05 mg/mL) 能够更有效地减少果蝇 AD 模型中的线粒体和细胞氧化应激[1]。LRP3-IN-33 (5 mg/kg; 腹腔注射; 一天一次连续 22 天) 能够改善东莨菪碱 (HY-N0296) (1.4 mg/kg; 腹腔注射; 一天一次连续 5 天) 诱导的 AD 小鼠模型的记忆障碍和认知障碍[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: |
| Formula | C21H19N3O5 |
| Molar Mass | 393.39 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Singh G, et al. Design, Synthesis, and Biological Evaluation of Ferulic Acid Template-Based Novel Multifunctional Ligands Targeting NLRP3 Inflammasome for the Management of Alzheimer's Disease. ACS Chem Neurosci. 2024;15(7):1388-1414. |