| Bioactivity | MQA-P is a multifunctional near-infrared (NIR) fluorescent probe for simultaneously detecting ONOO-, viscosity, and polarity within mitochondria. MQA-P exhibits a remarkable turn-on response to ONOO- (λem=645 nm) and is highly sensitive to viscosity/polarity in the NIR channel with λem>704 nm. MQA-P exhibits excited-state intramolecular charge transfer (ESICT) feature that is highly polarity-sensitive by engineering N,N-dimethylamino as the electron donor and a quinoline cationic unit as the electron acceptor. MQA-P is used for ferroptosis or cancer diagnosis in vitro and in vivo via dual-channel images[1]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. 将 MQA-P 溶解在二甲基亚砜 (DMSO) 中以制备储备液 (1.0 mM)。2. 用于活细胞中 ONOO- 的成像。HeLa 细胞与 MQA-P (5 μM) 孵育 30 分钟作为对照；用 SIN-1 (HY-126849; 100 μM) 预处理 30 分钟，然后用 MQA-P (5 μM) 再孵育 30 分钟。绿色通道 (λex=405 nm，λem=550-670 nm)。3. 活细胞黏度成像。HeLa 细胞与 MQA-P (5 μM) 孵育 30 分钟作为对照；用莫能菌素 (HY-N4302; 10 μM) 预处理 30 分钟，然后与 MQA-P (5 μM) 孵育 30 分钟。荧光图像使用共焦激光扫描显微镜，红色通道 (λex=561 nm，λem=680-750 nm)。4. 用于铁死亡过程中 ONOO-、粘度和极性的双通道成像。HeLa 细胞与 MQA-P (5 μM) 孵育 30 分钟作为对照；用 Erastin (HY-15763; 50 μM) 预处理 30 分钟，然后用 MQA-P (5 μM) 再孵育 30 分钟。荧光图像使用共聚焦激光扫描显微镜，绿色通道 (λex=405nm，λem=550-670 nm) 用于 ONOO-，红色通道 (λex=561 nm，λem=680-750 nm) 用于粘度和极性[1]。 |
| In Vivo | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. 对于组织切片成像，从小鼠身上分离出正常器官 (包括心脏、肝脏、脾脏、肺和肾脏) 和肿瘤，然后分别切成 5 μm 厚的切片。2. 这些切片用 MQA-P (20 μM) 孵育30分钟，然后用 PBS (pH 7.4) 洗涤 3 次，使用共聚焦激光扫描显微镜进行活体成像，绿色通道 (λex=405 nm, λem=550-670 nm) 用于 ONOO-，红色通道 (λex=561 nm, λem=680-750 nm) 用于粘度和极性[1]。 |
| Name | MQA-P |
| Formula | C40H36BrN2O2P |
| Molar Mass | 687.60 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Li Fan, et al. Multifunctional Fluorescent Probe for Simultaneous Detection of ONOO-, Viscosity, and Polarity and Its Application in Ferroptosis and Cancer Models. Anal Chem. 2023 Apr 4;95(13):5780-5787. |