| Bioactivity | HBT-Fl-BnB is a fluorescent probe for the ratiometric detection of ONOO- in vitro and in vivo. HBT-Fl-BnB consists of an HBT core with Fl groups at the ortho and para positions responding to the zwitterionic excited-state intramolecular proton-transfer (zwitterionic ESIPT) process and a boronic acid pinacol ester with dual roles that block the zwitterionic ESIPT and recognize ONOO-[1]. |
| Invitro | 指南 (以下是我们推荐的方案，本方案仅提供指南，应根据您的具体需要进行修改)。Raw264.7 细胞在细胞培养箱中继续加入不同浓度的 HBT-Fl-BnB 溶液 (0、5、10、15、20、30、50 μM)。 孵育24小时后，使用共焦显微镜的双通道对HBT-Fl-BnB处理的细胞进行成像：第一通道 (430ch) λem=415−515 nm，第二通道 (583ch) λex=405 nm，λem=550-650 nm[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> HBT-Fl-BnB 相关抗体: |
| In Vivo | 指南 (以下是我们推荐的方案，本方案仅提供指南，应根据您的具体需要进行修改)。选择八周大的雌性 C57BL/6 小鼠。成像前 30 分钟通过尾静脉注射 HBT-Fl-BnB (50 μM; 200 μL)。然后，使用小动物体内成像系统 PerkinElmer IVIS Lumina XR 和荧光镜导航器 360I 系统对这些小鼠进行成像。采用小动物体内双通道成像系统对 HBT-Fl-BnB 处理的小鼠进行成像：第一通道 (430ch) λem=415-515 nm，第二通道 (583ch) λex=405 nm 和 λem=550-650 nm[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
| Formula | C75H88BNO3S |
| Molar Mass | 1094.38 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Zhenkai WangA Fluorescent Probe with Zwitterionic ESIPT Feature for Ratiometric Monitoring of Peroxynitrite In Vitro and In Vivo. Anal Chem. 2024 Feb 27;96(8):3600-3608. |