| Bioactivity | Guanidine hydrochloride (Guanidinium chloride) a strong chaotrope, is also a strong denaturant of proteins[1][2]. |
| Invitro | Guanidine hydrochloride at low concentrations refolds acid-unfolded apomyoglobin and cytochrome c, stabilizing the molten globule state, i.e. a compact denatured state with a significant amount of secondary structure, but substantially disordered tertiary structure. Guanidine hydrochloride (> 1 M) causes co-operative unfolding of the molten globule state[1]. A considerable degree of ordered structure remains due to the presence of disulfide bonds, which Guanidine hydrochloride does not affect[2]. Guanidine hydrochloride at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine hydrochloride in growth media cures [PSI+] and other prions of yeast. 5 mM Guanidine hydrochloride significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine hydrochloride also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase[3]. |
| Name | Guanidine hydrochloride |
| CAS | 50-01-1 |
| Formula | CH6ClN3 |
| Molar Mass | 95.53 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |