| Bioactivity | Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ indicator. Storage: protect from light. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.1.First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.2.Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min.3.Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.4.Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.5.Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.6.Mount the coverslip on the imaging chamber. |
| Name | Fura-2 AM |
| CAS | 108964-32-5 |
| Formula | C44H47N3O24 |
| Molar Mass | 1001.85 |
| Appearance | Liquid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, protect from light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。 |
| Reference | [1]. Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23):1067. |
Fura-2 AM
CAS: 108964-32-5 F: C44H47N3O24 W: 1001.85
Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ indicator. Storage: pro
Sales Email：peptidedb@qq.com
This product is for research use only, not for human use. We do not sell to patients.