Bioactivity | Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm . Storage: protect from light. |
Invitro | A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1]. |
Name | Di-8-ANEPPS |
CAS | 157134-53-7 |
Formula | C36H52N2O3S |
Molar Mass | 592.87 |
Appearance | Solid |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | -20°C, protect from light *该产品在溶液状态不稳定,建议您现用现配,即刻使用。 |
Reference | [1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87. |