Flutax-2 (5/6-mixture)

CAS: F: C142H128F4N4O42 W: 1319.27

Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized

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Bioactivity	Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized α,β tubulin dimer. Excitation/emission wavelength: 496/524 nm. Paclitaxel, a diterpenoid secondary metabolite produced by Taxus species, can be used for the research of a variety of cancers[1]. Storage: protect from light.
Invitro	Flutax-2 工作液配制1.1 制备储存液用 DMSO 配制 10 mM 储备液。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 100 nM-1 μM 的 Flutax-2 工作液。注：请根据实际情况调整 Flutax-2 工作液浓度，且现用现配。2. 细胞染色2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 加入 1 mL Flutax-2 工作液，室温孵育 10-30 分钟。2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜进行观察。保存条件-20℃ 避光保存一年
Name	Flutax-2 (5/6-mixture)
Formula	C142H128F4N4O42
Molar Mass	1319.27
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	-20°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Reference	[1]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91. [2]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91.