Dabcyl-YVADAPV-EDANS_product_DataBase

Bioactivity	Dabcyl-YVADAPV-EDANS is a fluorogenic interleukin-1β converting enzyme (ICE) substrate for measuring ICE-like protease activity. Dabcyl-YVADAPV-EDANS detects fluorescence at 360 nm excitation and 480 nm emission wavelengths. ICE-like protease is a critical mediator of K+ deprivation-induced apoptosis of cerebellar granule neurons[1].
Invitro	ICE 活性测定方法[1]：(1) 将小脑颗粒神经元以 2.1*105/cm2 的密度接种在 24 孔板中。(2) DIV 8 状态的小脑颗粒神经元转换到含有低浓度 (5 mM) 或高浓度 (25 mM) 的 KCl 培养基中，用 Locke’s 缓冲液洗涤，并用 0.03% 的洋地黄苷 (Digitonin, HY-N4000) 使其通透。(3) 10 分钟后，加入 20 μM 的 DABCYL-YVADAPV-EDANS。(4) 在 24 孔板中使用 360 nm 激发和 480 nm 发射波长，1 小时内的荧光每间隔 10 分钟测定。(5) 荧光强度在 20 分钟达到峰值。该结果用于统计分析。
Name	Dabcyl-YVADAPV-EDANS
CAS	161877-70-9
Formula	C61H76N12O14S
Molar Mass	1233.39
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Schulz JB, et al. Potassium deprivation-induced apoptosis of cerebellar granule neurons: a sequential requirement for new mRNA and protein synthesis, ICE-like protease activity, and reactive oxygen species. J Neurosci. 1996 Aug 1;16(15):4696-706.

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