| Bioactivity | DDAO-C6 is a cridone ester derivative, highly specific fluorescence for detecting human serum albumin (HSA). DDAO-C6 acts as an enzymatic activatable near-infrared fluorescent probe in visually sensing endogenous lipase from gut microbes (Ex/Em=600/658 nm)[1][2]. Storage: protect from light. |
| Invitro | DDAO-C6 (10 μM; 30 min; 37 ℃) shows a linear relationship between the fluorescence intensities and lipase concentrations (0-50 μg/mL), and products a red fluorescence signal in the presence of active lipase[1].DDAO-C6 hydrolysates have fluorescence properties, with excitation wavelength=550-600 nm, emission spectrum=630-700 nm[2].Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Intestinal Fungi[1]:1. Disperse fresh intestinal secretions in sterile water and coate on a potato agar plate, containing penicillin (100 U/mL)/streptomycin (0.1 mg/mL) to inhibit intestinal bacteria. 2. Culture at 32 °C for about 5 days until the development of obvious fungal colonies. 3. Spray DDAO-C6 (100 μM) on colonies and incubate at 32 °C for 2 h. 4. Image plate samples on a Amersham Typhoon RGB (λex = 635 nm, λem = 670 ± 15 nm). |
| In Vivo | DDAO-C6 can be used to guide the rapid identification and cultivation of lipase active fungal strains from intestinal microbes in human feces, which is a potential technique for the biological investigation of intestinal microbes[2]. |
| Name | DDAO-C6 |
| CAS | 2102418-90-4 |
| Formula | C22H23Cl2NO3 |
| Molar Mass | 420.33 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Feng L, et al. Visual Identification of Trichosporon asahii, a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe. Anal Chem. 2022. [2]. Cui J, et al. Application of highly specific fluorescent probe for detection of human serum albumin: China, CN106841128[P]. 2017-06-13. |
DDAO-C6
CAS: 2102418-90-4 F: C22H23Cl2NO3 W: 420.33
DDAO-C6 is a cridone ester derivative, highly specific fluorescence for detecting human serum albumin (HSA). DDAO-C6 act
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