| Bioactivity | Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λem of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling[1][2]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Protein:1.The protein concentration was adjusted to 1 mg/mL for 100 μL.2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO (2 μL).3. Incubated overnight at 4 °C in the dark.4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate.5. Fluorescence intensities (FI) of labeled proteins were measured at excitation and emission wavelengths of 550 nm and 570 nm, respectively. |
| Name | Cyanine3 maleimide tetrafluoroborate |
| CAS | 2755154-93-7 |
| Formula | C36H43BF4N4O3 |
| Molar Mass | 666.56 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Zartner L, et al. Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments. J Mater Chem B. 2021 Nov 10;9(43):9012-9022. [2]. He J, et al. The effect of meat processing methods on changes in disulfide bonding and alteration of protein structures: impact on protein digestion products. RSC Adv. 2018 May 15;8(31):17595-17605. |
Cyanine3 maleimide tetrafluoroborate
CAS: 2755154-93-7 F: C36H43BF4N4O3 W: 666.56
Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λ
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