DAPI dihydrochloride_product_DataBase

Bioactivity	DAPI dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1].
Invitro	使用方法1. DAPI 工作液的配制1.1 制备储存液用 1 mL 双蒸水稀释 1 mg DAPI 去配制 1 mg/mL 的储备液。注：DAPI储存液建议分装后于 -20℃ 或 -80℃ 避光保存。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-10 μg/mL 的 DAPI 工作液。注：请根据实际情况调整 DAPI 工作液浓度，且现用现配。2. 细胞染色（悬浮细胞）2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在 1×106/mL2.2 加入 1 mL DAPI 工作液，室温孵育 3-10 分钟。2.3 400 g，离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。3. 细胞染色（贴壁细胞）3.1 将贴壁细胞培养于无菌盖玻片上。3.2 从培养基中移出盖玻片，吸除多余培养基。3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 3-10 分钟。3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟，使用荧光显微镜或流式细胞仪进行观察。保存条件-20℃ 避光保存一年注意事项1. 请根据实际情况调整 DAPI 工作液浓度与孵育时间。2. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Name	DAPI dihydrochloride
CAS	28718-90-3
Formula	C16H17Cl2N5
Molar Mass	350.25
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	-20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Reference	[1]. Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33. [2]. Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33.

PeptideDB

DAPI dihydrochloride

CAS: 28718-90-3 F: C16H17Cl2N5 W: 350.25