| Bioactivity | Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt. | ||||||
| Invitro | 16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2]. | ||||||
| Name | Crosstide | ||||||
| CAS | 171783-05-4 | ||||||
| Sequence | Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly | ||||||
| Shortening | GRPRTSSFAEG | ||||||
| Formula | C48H77N17O17 | ||||||
| Molar Mass | 1164.23 | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage | Sealed storage, away from moisture
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |