| Bioactivity | Cholera toxin (Choleragen), an AB(5)-subunit toxin, enters host cells by binding the ganglioside GM1 at the plasma membrane (PM) and travels retrograde through the trans-Golgi Network into the endoplasmic reticulum (ER)[1]. Choleragen activates adenylate cyclase by catalyzing ADP-ribosylation of Gs alpha, the stimulatory guanine nucleotide-binding protein[2]. |
| Invitro | 在 ER 中，Cholera toxin 的一部分，即酶促 A1 链，被蛋白质二硫键异构酶展开，并通过劫持错误折叠蛋白质的 ER 相关降解途径的组分逆向转运到胞质溶胶[1]。 Cholera toxin 充当 ADP-核糖基转移酶，破坏靶细胞中的细胞内信号传导。Cholera toxin 通过囊泡载体从细胞表面移动到中毒细胞的内质网 (ER)[3]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> Cholera toxin 相关抗体: |
| CAS | 9012-63-9 |
| Appearance | 固体 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Kellner A, et al. A binding motif for Hsp90 in the A chains of ADP-ribosylating toxins that move from the endoplasmic reticulum to the cytosol. Cell Microbiol. 2019;21(10):e13074. [2]. Tsai SC, et al. Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain. J Biol Chem. 1988;263(4):1768-1772. [3]. Wernick NL, et al. Cholera toxin: an intracellular journey into the cytosol by way of the endoplasmic reticulum. Toxins (Basel). 2010;2(3):310-325. |