Bioactivity | Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols[1][2]. Storage: protect from light. |
Invitro | Bromobimane (mBBr) 标记细胞中的反应性硫醇[1](1) 用乙腈配制 100 mM mBBr 溶液。(2) 15-25 µL 100 mM mBBr 溶液中加入 1 mL 10% 细胞悬液。(3) 37°C 孵育 30-45 min 后,进行荧光检测。 |
Name | Bromobimane |
CAS | 71418-44-5 |
Formula | C10H11BrN2O2 |
Molar Mass | 271.11 |
Appearance | Solid |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
Reference | [1]. Kosower EM, et al. Bromobimane probes for thiols. Methods Enzymol. 1995;251:133-48. [2]. Newton GL, et al. Determination of biothiols by bromobimane labeling and high-performance liquid chromatography. Methods Enzymol. 1995;251:148-66. |