| Bioactivity | Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Calculations:For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction: mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL BiotinBiotin Labeling Reaction:1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations. 2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO. 3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution. 4. Incubate reaction on ice for two hours or at room temperature for 30 minutes. 5. To tests the labeled protein by ELISA or Western blot. |
| Name | Biotin NHS |
| CAS | 35013-72-0 |
| Formula | C14H19N3O5S |
| Molar Mass | 341.38 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture and light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。 |
| Reference | [1]. Wojchowski DM et al. Hybridoma production by simplified avidin-mediated electrofusion. J Immunol Methods. 1986 Jun 24;90(2):173-7. [2]. Ngo TT et al. Biotinyl-glucose-6-phosphate dehydrogenase preparation, kinetics, and modulation by avidin. Appl Biochem Biotechnol. 1982 Nov;7(6):443-54. |