| Bioactivity | Boc-Lys(Ac)-AMC is a cell-permeable fluorometric HDAC substrate (Ex/Em = 355 nm/460 nm)[1][2]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 基于组织培养的 HDAC 检测[2]1. 将 6 × 104 细胞在 100 mL 培养基中加入 25 μM Boc-Lys(Ac)-AMC 于 96 孔组织培养板中培养过夜。2. 37°C，5% CO2 下孵育 2-3 小时。3. 加入含有细胞裂解缓冲液和胰蛋白酶试剂的 HADC 显影液，终止脱乙酰反应。4. 室温孵育 15 分钟。5. 在 Ex/Em = 355 nm/460 nm 处，用 SpectraMax M5 微板仪测量 AMC 的荧光信号。 |
| Name | Boc-Lys(Ac)-AMC |
| CAS | 233691-67-3 |
| Formula | C23H31N3O6 |
| Molar Mass | 445.51 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Reddy DS, et al. Measuring Histone Deacetylase Inhibition in the Brain. Curr Protoc Pharmacol. 2018 Jun;81(1):e41. [2]. Ueki N, et al. Selective cancer targeting with prodrugs activated by histone deacetylases and a tumour-associated protease. Nat Commun. 2013;4:2735. |