| Bioactivity | BRD7586 is a cell-permeable small-molecule inhibitor of SpCas9. BRD7586 is the smallest known anti-CRISPR[1]. | ||||||||||||
| Invitro | BRD7586在细胞中特异性地与 SpCas9 结合，但不与 Cas12a 结合[1]。BRD7586 (0-20 μM; 24 h) 在 HiBiT-knock-in 试验中，呈剂量依赖性地抑制 SpCas9[1]。BRD7586 (20 μM; 48 h) 增强 HEK293T 细胞中中靶与脱靶的比值[1]。 | ||||||||||||
| Name | BRD7586 | ||||||||||||
| CAS | 895460-70-5 | ||||||||||||
| Formula | C17H14ClN3O3S2 | ||||||||||||
| Molar Mass | 407.89 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
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| Reference | [1]. Lim D, et al. A general approach to identify cell-permeable and synthetic anti-CRISPR small molecules. Nat Cell Biol. 2022 Dec;24(12):1766-1775. |