Ac-LETD-AFC_product_DataBase

Bioactivity	Ac-LETD-AFC is a caspase-8 fluorogenic substrate. Ac-LETD-AFC can measure caspase-8 fluorogenic activity and can be used for the research of cancer cell apoptosis and oxidative stress metabolism[1].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Caspase activity assay[1]:1. Incubate the cells according to your normal protocol.2. Wash cells once with PBS and centrifugate.3. Resuspend cells in PBS at a concentration of 1× 107 cells/mL.4. Prepare the standard reaction buffer which includ 100 μM caspase-8 substrate AC-LETD-AFC. Prepare correspounding standard reaction buffer according to your protocol.5. Add 15 ul of the cells suspension to a microplate, and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer. 6. Measure substrate cleavage with a microplate reader with excitation wavelength of 360 nm and emission at 460 nm.
Name	Ac-LETD-AFC
CAS	210345-02-1
Formula	C31H38F3N5O12
Molar Mass	729.65
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.

PeptideDB