| Bioactivity | 5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together with an OsTIR1 (F74G) mutant. AID2 induces rapid and efficient depletion of mAID-fused proteins to study protein function in living cells, causing tumor suppression[1]. | ||||||||||||
| Invitro | HCT116 cells constitutively expressing OsTIR1(F74G) are exposed to 5-Ph-IAA (1 µM; 6 hours), leading to DHC1-mAC degradation, which means the AID2 system better than the original AID system in generating conditional mutant cell lines[1]. Western Blot Analysis[1] Cell Line: | ||||||||||||
| In Vivo | 5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system ,is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumour suppression[1]. Animal Model: | ||||||||||||
| Name | 5-Ph-IAA | ||||||||||||
| CAS | 168649-23-8 | ||||||||||||
| Formula | C16H13NO2 | ||||||||||||
| Molar Mass | 251.28 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
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| Reference | [1]. Aisha Yesbolatova, et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 2020 Nov 11;11(1):5701. |
5-Ph-IAA
CAS: 168649-23-8 F: C16H13NO2 W: 251.28
5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, establishes the auxin-inducible degron 2 (AID2) system together wit
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