Bioactivity | 2-Naphthylamine-6,8-disulfonic acid potassium is chemical that can be used for preparing dyestuff, medicine, agricultural chemicals etc[1]. |
Invitro | 2-Naphthylamine-6,8-disulfonic acid potassium (compound 20) can inhibit human immunodeficiency virus HIV-1 and HIV-2 by 11% and 13.6% at a concentration of 1369 μM, respectively[1].2-Naphthylamine-6,8-disulfonic acid potassium (ANDS) can directly analyze polyglycolic pyrophosphate-linked oligosaccharides in cell cultures and tissues[3].Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling oligosaccharides[3]:1. Prepared 0.15 M ANDS with 15% (v/v) acetic acid.2. Prepare 1M solution of sodium cyanoborohydride in dimethyl sulfoxide (DMSO).3. Add 5 μL ANDS reagent (1 μL if sample is below 200 pmol) to the sample.4. Add 5 μL sodium cyanoborohydride solution (1 μL if sample is below 200 pmol) to the sample.5. After mixing, the reaction was briefly centrifuged and allowed to dry for 18 hours at 37 °C. |
Name | 2-Naphthylamine-6,8-disulfonic acid potassium |
CAS | 842-15-9 |
Formula | C10H8KNO6S2 |
Molar Mass | 341.40 |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
Reference | [1]. G T Tan, et al. Potential anti-AIDS naphthalenesulfonic acid derivatives. Synthesis and inhibition of HIV-1 induced cytopathogenesis and HIV-1 and HIV-2 reverse transcriptase activities. J Med Chem. 1992 Dec 25;35(26):4846-53. [2]. K B Lee, et al. A new method for sequencing linear oligosaccharides on gels using charged, fluorescent conjugates. Carbohydr Res. 1991 Jul 18;214(1):155-68. [3]. Ningguo Gao, et al. Fluorophore-assisted carbohydrate electrophoresis: a sensitive and accurate method for the direct analysis of dolichol pyrophosphate-linked oligosaccharides in cell cultures and tissues. Methods. 2005 Apr;35(4):323-7. [4]. Preparation method of 2-naphthylamine 6,8 disulfonic acid. CN102295585A. |