Cy5 DBCO chloride_product_DataBase

Bioactivity	Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free “Click Chemistry” reaction[1]. Storage: protect from light.
Invitro	Cy5 DBCO chloride (2-50 μM) can control the extent of labelling by varying the concentration of DBCO-Cy5 in a dose-dependent manner, with the amount of DBCO-Cy5 bound at the cell surface increasing with increasing DBCO-Cy5 concentration[1].Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1.Incubate the cells at a density of 3×104 cells in a cell culture dish for 3 days and then wash the cells twice with DPBS (pH 7.4). Incubate the cells according to your normal protocol.2.Incubate the cells with Cy5 DBCO chloride at a final concentration of 20 µM for 1 h at 37°C.3.After incubation, wash cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combination fixative for 15 min at room temperature.4.After fixation, wash cells twice with DPBS (pH 7.4). Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
Name	Cy5 DBCO chloride
CAS	2182601-72-3
Formula	C50H53ClN4O2
Molar Mass	777.43
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Sun-Woong Kang, et al. Cell labeling and tracking method without distorted signals by phagocytosis of macrophages. Theranostics. 2014 Feb 12;4(4):420-31.

PeptideDB

Cy5 DBCO chloride

CAS: 2182601-72-3 F: C50H53ClN4O2 W: 777.43