Physicochemical Properties
| Molecular Formula | C19H11IN5NAO8S2 |
| Molecular Weight | 651.33 |
| Exact Mass | 650.899 |
| CAS # | 150849-52-8 |
| PubChem CID | 6099081 |
| Appearance | Light yellow to brown solid powder |
| Melting Point | > 300°C (dec.) |
| LogP | 4.216 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 10 |
| Rotatable Bond Count | 3 |
| Heavy Atom Count | 36 |
| Complexity | 942 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | JUJBNYBVVQSIOU-UHFFFAOYSA-M |
| InChi Code | InChI=1S/C19H12IN5O8S2.Na/c20-12-1-3-13(4-2-12)23-21-19(22-24(23)14-5-7-15(8-6-14)25(26)27)17-10-9-16(34(28,29)30)11-18(17)35(31,32)33/h1-11H,(H-,28,29,30,31,32,33)/q+1/p-1 |
| Chemical Name | Sodium 4-[2-(4-iodophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate |
| Synonyms | WST 1WST1 WST-1 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | WST-1 working solution composition 1.1 Prepare a stock solution of WST-1 containing 10 mg/mL of DMSO. For instance, dissolve 10 mg of WST-1 in 1 mL of DMSO. Note: The WST-1 storage solution should be aliquoted at -20°C. To prepare a 5–10 μM WST-1 working solution, mix the working solution with either PBS or serum-free cell culture medium that has already been prepared. Note: Please prepare WST-1 working fluid for usage by adjusting its concentration to suit the current circumstances. Cell staining 2.1 Suspension cell preparation: Centrifuge cells, add PBS, and wash twice for five minutes each time. Adherent cells: To break down the cells, discard the media and apply trypsin. Add PBS and centrifuge twice, for five minutes each time, to remove the supernatant after the first wash. 2.2 Stain for two hours after adding 1 mL of WST-1 working solution and 1 mM of 1-methoxy PMS. 2.3 Measure the OD at 450/690 nm using a microplate reader. |
| References |
[1]. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. 1993, 41(6): 1118-1122. |
Solubility Data
| Solubility (In Vitro) |
H2O : ~125 mg/mL (~191.91 mM) DMSO : ~25 mg/mL (~38.38 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.84 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5353 mL | 7.6766 mL | 15.3532 mL | |
| 5 mM | 0.3071 mL | 1.5353 mL | 3.0706 mL | |
| 10 mM | 0.1535 mL | 0.7677 mL | 1.5353 mL |