VUF10166 (VUF-10166; VUF 10166) is a novel, potent and competitive antagonist against serotonin 5-HT3A receptor with important biological activity. Its Ki is 0.04 nM, and it inhibits 5-HT3A.

Physicochemical Properties

Molecular Formula	C13H15CLN4
Molecular Weight	262.74
Exact Mass	262.098
Elemental Analysis	C, 59.43; H, 5.75; Cl, 13.49; N, 21.32
CAS #	155584-74-0
Related CAS #	155584-74-0
PubChem CID	24278976
Appearance	Light yellow to yellow solid powder
Density	1.3±0.1 g/cm3
Boiling Point	389.6±42.0 °C at 760 mmHg
Flash Point	189.4±27.9 °C
Vapour Pressure	0.0±0.9 mmHg at 25°C
Index of Refraction	1.637
LogP	2.79
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	18
Complexity	280
Defined Atom Stereocenter Count	0
SMILES	CN1CCN(C2=NC3=CC=CC=C3N=C2Cl)CC1
InChi Key	FFXVTQDGTKEXHF-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H15ClN4/c1-17-6-8-18(9-7-17)13-12(14)15-10-4-2-3-5-11(10)16-13/h2-5H,6-9H2,1H3
Chemical Name	2-chloro-3-(4-methylpiperazin-1-yl)quinoxaline
Synonyms	VUF 10166; VUF-10166; VUF10166
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	5-HT3A Receptor ( Ki = 0.04 nM ); 5-HT3 AB ( Ki = 22 nM )
ln Vitro	In vitro activity: VUUF10166 exhibits low 5-HT3AB receptor activity with Ki of 22 nM. VUF10166 may be used to can distinguish between 5-HT3A and 5-HT3AB receptors. VUF10166 exhibits no activity towards the α7 nACh receptor. Additionally, VUF10166 exhibits partial agonistic activity at 5-HT3A receptors, as evidenced by its EC50 of 5.2 μM, suggesting a low likelihood of binding within the receptor pore. [1]
ln Vivo
Animal Protocol
References	[1]. VUF10166, a novel compound with differing activities at 5-HT₃A and 5-HT₃AB receptors. J Pharmacol Exp Ther. 2012 May;341(2):350-9.

Solubility Data

Solubility (In Vitro)

DMSO: 21~100 mg/mL (79.9~380.6 mM) Water: <1 mg/mL Ethanol: ~61 mg/mL (~232.2 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8060 mL	19.0302 mL	38.0604 mL
	5 mM	0.7612 mL	3.8060 mL	7.6121 mL
	10 mM	0.3806 mL	1.9030 mL	3.8060 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.