UNC0646 (UNC-0646) is a novel, potent and selective histone methyltransferase G9a inhibitor with IC50 of 6 nM. UNC0646 had an outstanding tox/function ratio in MCF7 (470), 22RV1 (510), and IMR90 (360) cells, making this compound potentially more useful for investigating G9a biology in these specific cell lines. Compared to UNC0638, UNC0646 was more potent (IC50=6nM) and had a similar tox/function ratio in MDA-MB-231 cells (breast cancer cell). In addition, UNC0646 is highly selective for G9a/GLP (a G9a-like protein) over several other PKMTs (protein lysine methyltransferases) and PRMTs (protein arginine methyltransferases) including SETD7 (a H3K4 PKMT), SUV39H2 (a H3K9 PKMT), SETD8 (a H4K20 PKMT), and PRMT3.
Physicochemical Properties
| Molecular Formula | C36H59N7O2 |
| Molecular Weight | 621.8994 |
| Exact Mass | 621.473 |
| CAS # | 1320288-17-2 |
| PubChem CID | 53315882 |
| Appearance | White to light yellow solid powder |
| Density | 1.1±0.1 g/cm3 |
| Boiling Point | 754.6±70.0 °C at 760 mmHg |
| Flash Point | 410.1±35.7 °C |
| Vapour Pressure | 0.0±2.5 mmHg at 25°C |
| Index of Refraction | 1.587 |
| LogP | 5.75 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 9 |
| Rotatable Bond Count | 11 |
| Heavy Atom Count | 45 |
| Complexity | 845 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | OUKWLRHRXOPODD-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C36H59N7O2/c1-28(2)41-19-10-20-43(24-23-41)36-38-32-27-34(45-25-11-18-40-16-8-5-9-17-40)33(44-3)26-31(32)35(39-36)37-29-14-21-42(22-15-29)30-12-6-4-7-13-30/h26-30H,4-25H2,1-3H3,(H,37,38,39) |
| Chemical Name | N-(1-cyclohexylpiperidin-4-yl)-2-(4-isopropyl-1,4-diazepan-1-yl)-6-methoxy-7-(3-(piperidin-1-yl)propoxy)quinazolin-4-amine |
| Synonyms | UNC0646, UNC 0646, UNC-0646 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | In a variety of cell lines, UNC0646 (Compound 6) demonstrates strong cellular efficacy and a good separation between functional efficacy and cytotoxicity. UNC0646 exhibits minimal cytotoxicity and is quite successful at lowering H3K9me2 levels. UNC0646 in the following cell lines: IMR90, MDA-MB-231, MCF7, PC3, 22RV1, HCT116 wt, and HCT 116 p53-/- [1]. |
| References |
[1]. Optimization of cellular activity of G9a inhibitors 7-aminoalkoxy-quinazolines. J Med Chem. 2011 Sep 8;54(17):6139-50. |
| Additional Infomation | N-(1-cyclohexyl-4-piperidinyl)-6-methoxy-7-[3-(1-piperidinyl)propoxy]-2-(4-propan-2-yl-1,4-diazepan-1-yl)-4-quinazolinamine is a member of quinazolines. |
Solubility Data
| Solubility (In Vitro) |
DCM :≥ 50 mg/mL (~80.40 mM) DMSO : ~33.33 mg/mL (~53.59 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 4.55 mg/mL (7.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 45.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.02 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (4.02 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6080 mL | 8.0399 mL | 16.0798 mL | |
| 5 mM | 0.3216 mL | 1.6080 mL | 3.2160 mL | |
| 10 mM | 0.1608 mL | 0.8040 mL | 1.6080 mL |