Tridecanoic acid (N-Tridecanoic acid) is a 13-carbon medium-chain saturated fatty acid that could be utilized as an anti-adhesive and anti-biofilm agent to study bacterial infections. Tridecanoic acid inhibits E. coli persistence and biofilm formation.

Physicochemical Properties

Molecular Formula	C13H26O2
Molecular Weight	214.34
Exact Mass	214.193
CAS #	638-53-9
Related CAS #	Tridecanoic acid-d2;64118-44-1;Tridecanoic acid-d25;202529-03-1;Tridecanoic acid-d9
PubChem CID	12530
Appearance	White to off-white solid powder
Density	0.9±0.1 g/cm3
Boiling Point	308.2±5.0 °C at 760 mmHg
Melting Point	41-42 °C(lit.)
Flash Point	139.6±12.5 °C
Vapour Pressure	0.0±0.7 mmHg at 25°C
Index of Refraction	1.450
LogP	5.56
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	11
Heavy Atom Count	15
Complexity	144
Defined Atom Stereocenter Count	0
InChi Key	SZHOJFHSIKHZHA-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H26O2/c1-2-3-4-5-6-7-8-9-10-11-12-13(14)15/h2-12H2,1H3,(H,14,15)
Chemical Name	tridecanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Undecanoic acid, lauric acid, and N-tridecanoic acid inhibit Escherichia coli persistence and biofilm formation. J Microbiol Biotechnol. 2020 Oct 13.

Additional Infomation

Tridecanoic acid is a C13 straight-chain saturated fatty acid. It has a role as a plant metabolite. It is a long-chain fatty acid and a straight-chain saturated fatty acid. It is a conjugate acid of a tridecanoate. Tridecanoic acid has been reported in Leea guineensis, Inula grandis, and other organisms with data available. Tridecanoic acid is a short-chain fatty acid. See also: Fatty acids, C10-16 (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (466.55 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 6.25 mg/mL (29.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 6.25 mg/mL (29.16 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 6.25 mg/mL (29.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 900 μL corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.6655 mL	23.3274 mL	46.6548 mL
	5 mM	0.9331 mL	4.6655 mL	9.3310 mL
	10 mM	0.4665 mL	2.3327 mL	4.6655 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.