Testosterone sulfate pyridinium is a pyridine-containing testosterone sulfate. Testosterone sulfate is a metabolite of testosterone. Testosterone is the primary male sex hormone and its measurement helps assess androgenic status.

Physicochemical Properties

Molecular Formula	C24H33NO5S
Molecular Weight	447.59
CAS #	34991-10-1
Related CAS #	Testosterone sulfate;651-45-6
Appearance	White to off-white solid powder
SMILES	O=C1C=C2[C@]([C@@]3([C@@]([H])(CC2)[C@@]2([H])[C@]([C@H](CC2)OS(=O)(O)=O)(C)CC3)[H])(C)CC1.C1=CC=CC=N1
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Human Endogenous Metabolite
References	[1]. Pizzato EC, Filonzi M, Rosa HSD, de Bairros AV. Pretreatment of different biological matrices for exogenous testosterone analysis: a review. Toxicol Mech Methods. 2017;27(9):641-656. [2]. Niektóre problemy zwiazane z oznaczaniem testosteronu [Some problems with testosterone determination]. Endokrynol Pol. 2007;58(5):440-445.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (223.42 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.59 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (5.59 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2342 mL	11.1709 mL	22.3419 mL
	5 mM	0.4468 mL	2.2342 mL	4.4684 mL
	10 mM	0.2234 mL	1.1171 mL	2.2342 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.