Substance P(1-7) TFA is a fragment of the neuropeptide substance P (SP). Substance P(1-7) TFA exerts inhibitory and moderating effects on the nucleus of the solitary tract.

Physicochemical Properties

Molecular Formula	C43H66F3N13O12
Molecular Weight	1014.06
Exact Mass	1013.49
CAS #	2828433-22-1
Related CAS #	Substance P(1-7);68060-49-1
PubChem CID	134694976
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	12
Hydrogen Bond Acceptor Count	18
Rotatable Bond Count	27
Heavy Atom Count	71
Complexity	1760
Defined Atom Stereocenter Count	7
SMILES	C(N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@H](C(=O)O)CC1C=CC=CC=1)(=O)[C@H](CCCCN)NC([C@@H]1CCCN1C(=O)[C@@H](N)CCCNC(N)=N)=O.C(F)(F)(F)C(=O)O
InChi Key	NGJNGAVSGJSPNQ-OSSAIMHMSA-N
InChi Code	InChI=1S/C41H65N13O10.C2HF3O2/c42-19-5-4-12-28(51-37(60)30-13-7-21-53(30)38(61)25(43)11-6-20-48-41(46)47)39(62)54-22-8-14-31(54)36(59)50-27(16-18-33(45)56)34(57)49-26(15-17-32(44)55)35(58)52-29(40(63)64)23-24-9-2-1-3-10-24;3-2(4,5)1(6)7/h1-3,9-10,25-31H,4-8,11-23,42-43H2,(H2,44,55)(H2,45,56)(H,49,57)(H,50,59)(H,51,60)(H,52,58)(H,63,64)(H4,46,47,48);(H,6,7)/t25-,26-,27-,28-,29-,30-,31-;/m0./s1
Chemical Name	(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[(2S)-1-[(2S)-6-amino-2-[[(2S)-1-[(2S)-2-amino-5-(diaminomethylideneamino)pentanoyl]pyrrolidine-2-carbonyl]amino]hexanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoic acid;2,2,2-trifluoroacetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vivo

Following SP injection, substance P(1–7) is shown to develop locally in the nigra and to operate as a very powerful antagonist against the reactions elicited by SP. It has been suggested that Substance P(1–7) regulates SP activities endogenously[1]. When Substance P(1-7) (1.0-4.0 pM) is injected concurrently with SP or SP(5-11) (0.1 nM), the unpleasant behaviors caused by SP or SP(5-11) are greatly reduced. These findings suggest that endogenously produced SP(1–7) may be able to influence how SP or SP(5–11) behave in the spinal cord[2].

References

[1]. The substance P(1-7) fragment is a potent modulator of substance P actions in the brain. Brain Res. 1990 Jun 25;521(1-2):316-20. [2]. Substance P(1-7) antagonizes substance P-induced aversive behaviour in mice. Neurosci Lett. 1988 Dec 19;95(1-3):281-5.

Solubility Data

Solubility (In Vitro)	H2O: 50 mg/mL (49.31 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (98.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.9861 mL	4.9307 mL	9.8613 mL
	5 mM	0.1972 mL	0.9861 mL	1.9723 mL
	10 mM	0.0986 mL	0.4931 mL	0.9861 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.