Saccharin sodium hydrate is an orally bioavailable, non-caloric artificial sweetener (NAS). Saccharin sodium hydrate has bacteriostatic (inhibition of bacterial growth) and microbial modulating properties.

Physicochemical Properties

Molecular Formula	C7H6NNAO4S
Molecular Weight	223.1816
Exact Mass	241.002
CAS #	82385-42-0
Related CAS #	Saccharin;81-07-2
PubChem CID	23675258
Appearance	Typically exists as solid at room temperature
Boiling Point	438.9ºC at 760 mmHg
Melting Point	>300°C
Flash Point	219.3ºC
LogP	1.185
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	0
Heavy Atom Count	13
Complexity	319
Defined Atom Stereocenter Count	0
SMILES	[Na].O=C1C2C(=CC=CC=2)S(=O)(=O)N1.O
InChi Key	AYGJDUHQRFKLBG-UHFFFAOYSA-M
InChi Code	InChI=1S/C7H5NO3S.Na.2H2O/c9-7-5-3-1-2-4-6(5)12(10,11)8-7;;;/h1-4H,(H,8,9);;2*1H2/q;+1;;/p-1
Chemical Name	sodium;1,1-dioxo-1,2-benzothiazol-2-id-3-one;dihydrate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Bacterial growth is inhibited by saccharin sodium hydrate (0.5, 2.5, and 5 mM) in vitro in a species-dependent way [1].
ln Vivo	Male C57BL/6JRj wild-type (wt) mice's intestinal barrier is not considerably impacted by the oral administration of saccharin sodium hydrate (5 mg/kg; twice daily), which decreases the bacterial load in their feces and modifies the composition of their microbiome [1].
References	[1]. Sünderhauf A, et al. Saccharin Supplementation Inhibits Bacterial Growth and Reduces Experimental Colitis in Mice. Nutrients. 2020 Apr 17;12(4). pii: E1122.
Additional Infomation	Saccharin, sodium salt appears as odorless white crystals or crystalline powder. Aqueous solution is neutral or alkaline to litmus, but not alkaline to phenolphthalein. Effloresces in dry air. Intensely sweet taste. (NTP, 1992) Flavoring agent and non-nutritive sweetener.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL H2O : ~83.33 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 100 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.4807 mL	22.4034 mL	44.8069 mL
	5 mM	0.8961 mL	4.4807 mL	8.9614 mL
	10 mM	0.4481 mL	2.2403 mL	4.4807 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.