Physicochemical Properties
| Molecular Formula | C13H15CL2F3N4O5PTS |
| Appearance | White to off-white solid |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | SLC7A11[1] |
| ln Vitro | Compound 2, SLC7A11-IN-1, has anti-proliferative action against HCT-116, MDA-MB-231, MCF-7, HepG2, and LO2 cells, with IC50 values of 0.03 and 0.11, 0.18, 0.17, and 0.27 µM, respectively, during a 72-hour period [1]. SLC7A11-IN-1 (0.5 µM; 24 h) prevents HCT-116 cells from invasively growing and metastasizing [1]. In S phase, SLC7A11-IN-1 (0.5 µM; 24 h) causes cell cycle arrest and apoptosis [1]. Reactive oxygen species (ROS) expression is markedly upregulated and intracellular GSH concentration is considerably decreased by SLC7A11-IN-1 [1]. In HCT-116 cells, SLC7A11-IN-1 (1 µM; 15 h) dramatically increases DNA damage and associated protein expression [1]. |
| ln Vivo | SLC7A11-IN-1 (2 mg/kg; iv; 3 times every three days) prevents the development of cancers in mice[1] |
| Cell Assay |
Cell Cycle Analysis[1] Cell Types: HCT-116 cells Tested Concentrations: 0.5 µM Incubation Duration: 24 h Experimental Results: Induced cell apoptosis and cell cycle arrest at S-phase with the S-phase proportion of 55.79%. Western Blot Analysis[1] Cell Types: HCT-116 cells Tested Concentrations: 1 µM Incubation Duration: 15 h Experimental Results: Increased the expression of p53, Bax, p-Akt, VEGFA and HIF1-α proteins, diminished the expression of Bcl-2, xCT protein level. |
| Animal Protocol |
Animal/Disease Models: male balb/c (Bagg ALBino) mouse (CT26 tumor models)[1] Doses: 2 mg/ kg Route of Administration: Iv; every three days for consecutive 3 times Experimental Results: Suppressed the growth of tumor by 60.7%. |
| References | [1]. Li Z, et al. Blocking xCT and PI3K/Akt pathway synergized with DNA damage of Riluzole-Pt(IV) prodrugs for cancer treatment. Eur J Med Chem. 2023 Mar 15;250:115233. |
Solubility Data
| Solubility (In Vitro) | DMSO :≥ 20 mg/mL (~30.20 mM) |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |