RSK4-IN-1 TFA is a potent RSK4 inhibitor with IC50 of 9.5 nM. RSK4-IN-1 TFA exhibits antitumor activity.

Physicochemical Properties

Molecular Formula	C21H21F5N4O5
Molecular Weight	504.41
Appearance	Solid Powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	RSK4 9.5 nM (IC50)
References	[1]. Yuan Y, et al. Discovery, Optimization, and Structure-Activity Relationship Study of Novel and Potent RSK4 Inhibitors as Promising Agents for the Treatment of Esophageal Squamous Cell Carcinoma. J Med Chem. 2021 Sep 23;64(18):13572-13587.

Solubility Data

Solubility (In Vitro)	DMSO : ~100 mg/mL (~198.25 mM; with ultrasonication (<80°C))
Solubility (In Vivo)	Solubility in Formulation 1: 2.5 mg/mL (4.96 mM) in 10% DMSO +40% PEG300 +5% Tween-80 +45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication。 Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9825 mL	9.9126 mL	19.8251 mL
	5 mM	0.3965 mL	1.9825 mL	3.9650 mL
	10 mM	0.1983 mL	0.9913 mL	1.9825 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.