Pseudouridimycin TFA is an antibiotic and a selective bacterial RNA polymerase (RNAP) inhibitor with an IC50 of approximately 0.1 μM and a minimum inhibitory concentration (MIC) of 4 - 6 μg/mL. Pseudouridimycin TFA is a C-nucleoside analog that is active against both Gram-negative and Gram-positive bacteria. Pseudouridimycin TFA inhibits bacterial growth in vitro and is active in a mouse peritonitis model of infection with Streptococcus pyogenes.

Physicochemical Properties

Molecular Formula	C19H27F3N8O11
Molecular Weight	600.46
CAS #	2760807-99-4
Synonyms	PUM TFA
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Discovery, properties, and biosynthesis of pseudouridimycin, an antibacterial nucleoside-analog inhibitor of bacterial RNA polymerase. J Ind Microbiol Biotechnol. 2019 Mar;46(3-4):335-343.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.6654 mL	8.3269 mL	16.6539 mL
	5 mM	0.3331 mL	1.6654 mL	3.3308 mL
	10 mM	0.1665 mL	0.8327 mL	1.6654 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.