Naringinase is a hydrolase complex with α-L-rhamnosidase and β-D-glucosidase activities. Naringinase occurs widely in nature. Naringinase can be used for the biotransformation of steroids and antibiotics, mainly for the hydrolysis of glycosides.

Physicochemical Properties

Molecular Formula	C11H9N3O2.NA+
Molecular Weight	238.19786
CAS #	9068-31-9
Appearance	White to off-white solid powder
SMILES	[Na+].O=C1C=C/C(=N/NC2=CC=CC=N2)/C(O)=C1
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In the processing of oranges and grapefruits, naringinase is used to enhance pulp washing, boost essential oil recovery, and debitter and clarify the juice [1]. Glycopeptide antibiotics, flavonoids, and glycolipids can all be deglycosylated with naringinase [2].
References	[1]. Production, purification, and characterization of the debittering enzyme naringinase. Biotechnol Adv. 2000 May;18(3):207-17. [2]. Ribeiro MH. Naringinases: occurrence, characteristics, and applications. Appl Microbiol Biotechnol. 2011 Jun;90(6):1883-95.

Solubility Data

Solubility (In Vitro)	H2O: 120 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 33.33 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.1982 mL	20.9908 mL	41.9815 mL
	5 mM	0.8396 mL	4.1982 mL	8.3963 mL
	10 mM	0.4198 mL	2.0991 mL	4.1982 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.