Mevalonic acid (MVA) lithium salt is a precursor of the mevalonate pathway and is essential for cell growth and proliferation. Mevalonic acid lithium salt can effectively inhibit the decrease in C2C12 cell viability induced by Simvastatin in vitro and can be used for the study of myopathy and heart failure.

Physicochemical Properties

Molecular Formula	C6H13LIO4
Molecular Weight	156.106022596359
Exact Mass	154.081
CAS #	2618458-93-6
Related CAS #	Mevalonic acid;150-97-0
PubChem CID	76224943
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	4
Heavy Atom Count	11
Complexity	128
Defined Atom Stereocenter Count	0
SMILES	C(C(=O)O)C(O)(C)CCO.[LiH]
InChi Key	PVWNXFFXFNEHDZ-UHFFFAOYSA-M
InChi Code	InChI=1S/C6H12O4.Li/c1-6(10,2-3-7)4-5(8)9;/h7,10H,2-4H2,1H3,(H,8,9);/q;+1/p-1
Chemical Name	lithium;3,5-dihydroxy-3-methylpentanoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Mevalonic acid lithium salt (80, 90, 100, and 110 µM; 72 hours) prevents the loss of viability of C2C12 myotube cells caused by simvastatin in vitro[1].
Cell Assay	Cell Viability Assay[1] Cell Types: C2C12 cells ( simvastatin-induced) Tested Concentrations: 80, 90, 100, 110 µM Incubation Duration: 72 h Experimental Results: demonstrated no decline in cell viability.
References	[1]. Moschetti A, et al. Coenzyme Q nanodisks counteract the effect of statins on C2C12 myotubes. Nanomedicine. 2021 Oct;37:102439. [2]. Soma MR, et al. Cholesterol and mevalonic acid modulation in cell metabolism and multiplication. Toxicol Lett. 1992 Dec;64-65 Spec No:1-15.

Solubility Data

Solubility (In Vitro)

H2O :~100 mg/mL (~648.97 mM) DMSO :~50 mg/mL (~324.49 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (16.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.22 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (16.22 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 100 mg/mL (648.97 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.4057 mL	32.0287 mL	64.0574 mL
	5 mM	1.2811 mL	6.4057 mL	12.8115 mL
	10 mM	0.6406 mL	3.2029 mL	6.4057 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.