M04 is a STING agonist. M04 induces expression of an IFN reporter gene in HEK293T cells expressing wild-type human STING, but not in HEK293T cells expressing the R71H-G230A-R293Q (HAQ) STING variant and in mouse RAW 264.7 cells, indicating that its activity is allele- and species-dependent. M04 induces the production of TNF-α, IL-10, IL-1β, and IL-12p70 in human peripheral blood mononuclear cells (PBMCs). At a concentration of 50 µM, M04 stimulates dendritic cells isolated from PBMCs to express the MHC class II cell surface receptor HLA-DR and the co-stimulatory molecules CD40, CD80, and CD86, and also enhances the ability of these cells to activate T cells in vitro. M04 can be used to study inflammatory immune diseases.

Physicochemical Properties

Molecular Formula	C18H24N2O4S3
Molecular Weight	428.59
CAS #	875158-73-9
SMILES	S1C(N(C)C)=C(S(C2=CC=C(C)C=C2)(=O)=O)N=C1S(C1CCCCC1)(=O)=O
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Characterization of a Novel Compound That Stimulates STING-Mediated Innate Immune Activity in an Allele-Specific Manner. Front Immunol. 2020 Jul 8;11:1430.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3332 mL	11.6662 mL	23.3323 mL
	5 mM	0.4666 mL	2.3332 mL	4.6665 mL
	10 mM	0.2333 mL	1.1666 mL	2.3332 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.