L-Aspartic acid-13C is 13C (carbon 13) labelled L-Aspartic acid. L-Aspartic acid is an amino acid (AA) that has been shown to be a precursor active molecule for colon-specific active molecule delivery.

Physicochemical Properties

Molecular Formula	C313CH7NO4
Molecular Weight	134.10
Exact Mass	134.04
CAS #	81201-97-0
Related CAS #	L-Aspartic acid;56-84-8
PubChem CID	16213451
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Melting Point	>300ºC (dec.)(lit.)
Index of Refraction	1.531
LogP	-2.8
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	9
Complexity	133
Defined Atom Stereocenter Count	1
SMILES	[C@@H](N)([13C](=O)O)CC(=O)O
InChi Key	CKLJMWTZIZZHCS-GZPBOPPUSA-N
InChi Code	InChI=1S/C4H7NO4/c5-2(4(8)9)1-3(6)7/h2H,1,5H2,(H,6,7)(H,8,9)/t2-/m0/s1/i4+1
Chemical Name	(2S)-2-amino(113C)butanedioic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

It has been demonstrated that L-aspartic acid is an appropriate prodrug for colon-specific drug delivery[1]. Preadministration of 100 mM L-Aspartic acid and 100 mM L-Glu increases the amount of L-[3H]Asp that is still in the brain at 5 minutes by 206 and 178%, respectively; L-[3H]Asp efflux transport is unaffected by 100 mM D-Asp. The coadministration of 100 mM L-Aspartic acid and 100 mM L-Glu, respectively, increases that value for L-[3H]Glu at 20 minutes by 145 and 156%, but not by D-Asp. It's noteworthy to note that L-Aspartic acid appears to traverse the BBB seven times faster than L-Glu does[2].

References

[1]. In vivo pharmacokinetic study for the assessment of poly(L-aspartic acid) as a drug carrier for colon-specific drug delivery. J Pharmacokinet Biopharm. 1995 Aug;23(4):397-406. [2]. Blood-brain barrier produces significant efflux of L-aspartic acid but not D-aspartic acid: in vivo evidence using the brain efflux index method. J Neurochem. 1999 Sep;73(3):1206-11.

Solubility Data

Solubility (In Vitro)	H2O: 5 mg/mL (37.29 mM) DMSO: < 1 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 2 mg/mL (14.91 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	7.4571 mL	37.2856 mL	74.5712 mL
	5 mM	1.4914 mL	7.4571 mL	14.9142 mL
	10 mM	0.7457 mL	3.7286 mL	7.4571 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.