Jedi2 is a Piezo1 activator, but not a Piezo2 activator. Jedi2 binds to mouse Piezo1 protein with Kd of 2770 μM.

Physicochemical Properties

Molecular Formula	C10H8O3S
Molecular Weight	208.23372
Exact Mass	208.019
CAS #	651005-90-2
PubChem CID	2796026
Appearance	Light brown to brown solid powder
LogP	3.014
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	2
Heavy Atom Count	14
Complexity	231
Defined Atom Stereocenter Count	0
InChi Key	YXDCRSXNEOKXDE-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H8O3S/c1-6-7(10(11)12)5-8(13-6)9-3-2-4-14-9/h2-5H,1H3,(H,11,12)
Chemical Name	2-methyl-5-thiophen-2-ylfuran-3-carboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Piezo1-mediated Ca2+ influx is caused by Jedi2. In mPiezo1-transfected cells, Jedi2 (200 μM) potential elicited quantifiable responses, but not in mPiezo2- or vector-transfected cells [1]. In mCherry HEK293T cells transfected with the mPiezo1-mCherry construct, Jedi2 (1 mM) dramatically increases Ca2+ levels; these cells are similarly responsive to Yoda1 (30 μM) [1].
References	[1]. A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel. Nat Commun. 2018 Apr 3;9(1):1300.
Additional Infomation	2-Methyl-5-(2-thienyl)-3-furoic acid is a furoic acid.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~240.12 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (12.01 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.8024 mL	24.0119 mL	48.0238 mL
	5 mM	0.9605 mL	4.8024 mL	9.6048 mL
	10 mM	0.4802 mL	2.4012 mL	4.8024 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.