Diethylnorspermine 4HCl (DENSPM, BE-333), the tetrahydrochloride salt of Diethylnorspermine, is a potent inducer of spermidine/spermine N1-acetyltransferase, which is a polyamine catabolic enzyme. It is a polyamines analog that is able to induce cell cycle arrest, inhibit proliferation and induce apoptosis in melanoma, breast, prostate, lung and colon cancer cells.

Physicochemical Properties

Molecular Formula	C13H32N4
Molecular Weight	244.43
Exact Mass	388.169
CAS #	156886-85-0
Related CAS #	N1,N11-Diethylnorspermine;121749-39-1
PubChem CID	11501988
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	8
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	14
Heavy Atom Count	21
Complexity	117
Defined Atom Stereocenter Count	0
InChi Key	LAWBSOKBIROCQP-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H32N4.4ClH/c1-3-14-8-5-10-16-12-7-13-17-11-6-9-15-4-2;;;;/h14-17H,3-13H2,1-2H3;4*1H
Chemical Name	N-ethyl-N'-[3-[3-(ethylamino)propylamino]propyl]propane-1,3-diamine;tetrahydrochloride
Synonyms	BE-333 HCl BE333 HClBE 333 HCl DE333 BE 3-3-3 BE-333 tetrahydrochloride DE-333DE 333 BE333BE 333 BE-333N(1),N(11)-DiethylnorspermineDENSPMBENSPM N1,N11-Bis(ethyl)norspermineDiethyl-norspermine N1,N11-Diethylnorspermine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Structural basis for differential induction of spermidine/spermine N1-acetyltransferase activity by novel spermine analogs. Mol Pharmacol. 1997 Jul;52(1):69-74.

Solubility Data

Solubility (In Vitro)	H2O : ~33.33 mg/mL (~85.40 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (256.24 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.0912 mL	20.4558 mL	40.9115 mL
	5 mM	0.8182 mL	4.0912 mL	8.1823 mL
	10 mM	0.4091 mL	2.0456 mL	4.0912 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.